Maintaining Integrity: Recommended Storage and Handling Procedures for the 96 Well 0.2ml Double Colour Full Skirt PCR Plate

PCR (Polymerase Chain Reaction) plates are crucial components in molecular biology and genetic research. To ensure accurate and reliable results, it is essential to maintain the integrity of the 96 Well 0.2ml Double Colour Full Skirt PCR Plate through proper storage and handling. In this blog, we will outline the recommended procedures to preserve the functionality and performance of these specialized PCR plates.

1. Proper Storage Conditions

Temperature Control:

- Store the PCR plates at room temperature (15-25°C) in a dry environment. Extreme temperatures can warp or damage the plastic, compromising the plate’s structural integrity.

- Avoid exposing the plates to direct sunlight or heat sources, as UV radiation and excessive heat can degrade the material over time.

Humidity Control:

- Maintain a low-humidity environment for storage to prevent moisture absorption, which can affect the plate's performance.

- Use desiccants in storage containers to control humidity levels and protect the plates from moisture damage.

Clean Storage Area:

- Store the plates in a clean, dust-free area to prevent contamination. Dust and debris can interfere with PCR reactions and lead to inaccurate results.

- Use sealed containers or bags to protect the plates from environmental contaminants.

2. Handling Procedures

Minimize Physical Stress:

- Handle the plates with care to avoid bending or warping. Use both hands to support the plate when carrying it to distribute the weight evenly.

- Avoid stacking heavy objects on top of the plates, which can cause deformation and damage the wells.

Use Gloves and Protective Gear:

- Always wear gloves when handling PCR plates to prevent contamination from skin oils, dirt, or DNA. This is crucial for maintaining the sterility of the wells.

- Consider wearing a lab coat and using clean, dedicated tools to further reduce the risk of contamination.

Labeling and Identification:

- Clearly label the plates with a permanent marker or lab-specific labeling system to ensure proper identification and avoid mix-ups.

- Use the double-color design to organize and track samples efficiently, minimizing handling errors.

3. Transport Guidelines

Secure Packaging:

- When transporting PCR plates, use sturdy, shock-absorbent packaging to protect against physical damage. Bubble wrap or foam inserts can provide additional cushioning.

- Ensure the plates are secured within the packaging to prevent movement and potential damage during transit.

Temperature and Humidity Control:

- Maintain appropriate temperature and humidity conditions during transport to prevent exposure to extremes that could compromise the plates.

- Use insulated containers or coolers with gel packs if temperature control is necessary, particularly for long-distance transportation.

4. Quality Assurance and Inspection

Regular Inspections:

- Periodically inspect stored PCR plates for signs of damage or contamination. Check for any warping, discoloration, or visible particles in the wells.

- Discard any compromised plates to prevent unreliable PCR results.

Documentation and Inventory Management:

- Keep detailed records of plate batches, including purchase dates, storage conditions, and usage history. This helps in tracking potential issues and ensures proper inventory management.

- Implement a first-in, first-out (FIFO) system to use older plates before newer ones, reducing the risk of material degradation over time.

5. Best Practices for Use

Pre-Use Inspection:

- Before using a PCR plate, conduct a visual inspection to ensure it is free from defects or contamination. Confirm that the plate is clean and intact.

- Check the integrity of the wells and the skirt, ensuring there are no cracks or warping that could affect the PCR process.

Consistent Protocols:

- Follow consistent protocols for pipetting, sealing, and thermal cycling to maintain the integrity of the PCR plate throughout the experiment.

- Use the double-color design to aid in well identification and reduce the likelihood of cross-contamination or pipetting errors.

Conclusion

Proper storage and handling of the 96 Well 0.2ml Double Colour Full Skirt PCR Plate are essential for maintaining its integrity and ensuring reliable PCR results. By adhering to recommended temperature and humidity conditions, handling with care, securing plates during transport, conducting regular inspections, and following best practices for use, researchers can preserve the functionality of these specialized plates. Implementing these procedures not only enhances the accuracy and reproducibility of experiments but also extends the lifespan of the PCR plates, contributing to more efficient and effective laboratory operations.